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KMID : 0811720120160050361
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Korean Journal of Physiology & Pharmacology
2012 Volume.16 No. 5 p.361 ~ p.365
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Gecko Proteins Exert Anti-Tumor Effect against Cervical Cancer Cells Via PI3-Kinase/Akt Pathway
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Jeong Ae-Jin
Chung Chung-Nam
Kim Hye-Jin
Bae Kil-Soo
Choi Song
Jun Woo-Jin
Shim Sang-In
Kang Tae-Hong
Leem Sun-Hee
Chung Jin-Woong
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Abstract
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Anti-tumor activity of the proteins from Gecko (GP) on cervical cancer cells, and its signaling mechanisms were assessed by viable cell counting, propidium iodide (PI) staining, and Western blot analysis. GP induced the cell death of HeLa cells in a dose-dependent manner while it did not affect the viability of normal cells. Western blot analysis showed that GP decreased the activation of Akt, and co-administration of GP and Akt inhibitors synergistically exerted anti-tumor activities on HeLa cells, suggesting the involvement of PI3-kinase/Akt pathway in GP-induced cell death of the cancer cells. Indeed, the cytotoxic effect of GP against HeLa cells was inhibited by overexpression of constituvely active form of Akt in HeLa cells. The candidates of the functional proteins in GP were analyzed by Mass-spectrum. Taken together, our results suggest that GP elicits anti-tumor activity against HeLa cells by inhibition of PI3-kinase/Akt pathway.
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KEYWORD
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Cervical cancer, Gecko, Lizard, PI3-kinase, Tumor
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